Characterization of a mutant human erythrocyte carbonic anhydrase: Carbonic anhydrase IcGuam
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منابع مشابه
Carbonic anhydrase in human platelets.
The carbonic anhydrase activity of human platelets was investigated by measuring the kinetics of CO2 hydration in supernatants of platelet lysates by using a pH stopped-flow apparatus. An average carbonic anhydrase concentration of 2.1 microM was determined for pellets of human platelets. Analysis of the kinetic properties of this carbonic anhydrase yielded a Km value of 1.0 mM, a catalytic-cen...
متن کاملCharacterization of a mutant human erythrocyte carbonic anhydrase: carbonic anhydrase Ic-Guam. The amino acid substitution and carboxylesterase and hydratase activities.
Evidence is presented which indicates that an arginine residue has substituted for a glycine residue in the abnormal human erythrocyte carbonic anhydrase, CA Ic ouam. The ratio of the amount of mutant to normal enzyme isolated from hemolyzates of individuals heterozygous for the variant averaged 0.56:1. No significant differences were observed between the specific carboxylesterase activities or...
متن کاملCarbonic anhydrase under pressure
Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.
متن کاملGastric Carbonic Anhydrase In
Davenport and Fisher (1938) reported the discovery DOGS P School of Medicine of large amounts of carbonic anhydrase in the gastric mucosa of cats, rats and rabbits. Davenport (1939) demonstrated that carbonic anhydrase is present in high concentration in the parietal cells and in lower concentration in the cells of the surface epithelium of the gastric mucosa of cats and rats. When those invest...
متن کاملDistribution of carbonic anhydrase among human photoreceptors.
The distribution of carbonic anhydrase (CA) among human photoreceptors has been a topic of dispute. In our experiments, by modifying an established enzyme histochemical technique, reproducible staining was observed. Of the cones in the peripheral retina, 91% were positive for CA. The CA-negative (CA-) cones were absent within approximately 8 arc min of the foveal center and their density peaked...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 1966
ISSN: 0003-9861
DOI: 10.1016/0003-9861(66)90419-x